Toxic Effects of Prenylated Flavone Compounds from the Chloroform Fraction of Kaledang (Artocarpus Lanceifolius Roxb.) Stem Bark In Vitro

Abstract

Artocarpus is a genus in the Moraceae family that contains many prenylated flavonoid compounds. This study aimed to isolate prenylated flavonoid compounds from the chloroform fraction of the stem bark of Artocarpus lanceifolius Roxb. The extraction method used was multistage maceration, using solvents based on increasing polarity. Fractionation was performed using Vacuum Column Chromatography (VCCh), isolation using Radial Chromatography (CR), and structure elucidation using UV-Vis spectrophotometers, FTIR, H-NMR, C-NMR, and two-dimensional NMR (HMBC and HSQC). Toxicity testing was carried out using the Brine Shirimp Lethality Test (BSLT) using Artemia salina Leach. The isolation results indicated the presence of a prenylated flavone compound, Artobilosanthone. The toxicity test for Artobilosanthone showed an LC50 value of 3.36 µg/mL, making it highly toxic.